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- Title
The structure of CMS2MS2, a mitogenic protein isolated from Carica candamarcensis.
- Authors
Gomes, Marco Túlio R.; Bemquerer, Marcelo P.; Lopes, Miriam Tereza P.; Richardson, Michael; Oyama Júnior, Sergio; Salas, Carlos E.
- Abstract
In a recent study we showed that two proteinases (CMS2MS2 and CMS2MS3) from Carica candamarcensis enhance mammalian cell proliferation. The aim of the present study is the determination of the primary structure of CMS2MS2 and prediction of its three-dimensional structure. The protein contains 214 residues, including the catalytic triad composed of Cys25, His159, and Asn175. A phylogenetic tree analysis demonstrated that CMS2MS2 ranks closer to chymopapain than to papain. The overall predicted three-dimensional structure is similar to proteinases from the papain family. These results suggest that minor structural differences within CMS2MS2 must account for its proliferative action.
- Subjects
CELL proliferation; CARICACEAE; CYSTEINE proteinases; CHYMOPAPAIN; PAPAIN
- Publication
Biological Chemistry, 2007, Vol 388, Issue 8, p819
- ISSN
1431-6730
- Publication type
Article
- DOI
10.1515/BC.2007.083