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- Title
Polyoxometalate functionalized magnetic metal–organic framework with multi-affinity sites for efficient enrichment of phosphopeptides.
- Authors
Jiang, Dandan; Qi, Ruixue; Wu, Siyu; Li, Yangyang; Liu, Jinghai
- Abstract
The reasonable design of metal–organic framework (MOF)–derived nanomaterial has important meaning in increasing the enrichment efficiency in the study of protein phosphorylation. In this work, a polyoxometalate (POM) functionalized magnetic MOF nanomaterial (Fe3O4@MIL-125-POM) was designed and fabricated. The nanomaterial with multi-affinity sites (unsaturated metal sites and metal oxide clusters) was used for the enrichment of phosphopeptides. Fe3O4@MIL-125-POM had high-efficient enrichment performance towards phosphopeptides (selectivity, a mass ratio of bovine serum albumin/α-casein/β-casein at 5000:1:1; sensitivity, 0.1 fmol; satisfactory repeatability, ten times). Furthermore, Fe3O4@MIL-125-POM was employed to enrich phosphopeptides from non-fat milk digests, saliva, serum, and A549 cell lysate. The enrichment results illustrated the great potential of Fe3O4@MIL-125-POM for efficient identification of low-abundance phosphopeptides.
- Subjects
METAL-organic frameworks; PHOSPHOPEPTIDES; METAL clusters; CASEINS; METALLIC oxides; NANOSTRUCTURED materials; SERUM albumin; TRANSCRANIAL magnetic stimulation
- Publication
Analytical & Bioanalytical Chemistry, 2024, Vol 416, Issue 19, p4289
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-024-05365-y