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- Title
Optimization condition in labeling of Ofloxacin with <sup>99m</sup>TC and its biological evaluation in Staphylococcus aureus and Escherichia coli for infection imaging.
- Authors
Erfani, Mostafa; Doroudi, Alireza; Hadisi, Leila; Andishmand, Ali; Mohammad Mazidi, Seyed
- Abstract
Introduction: The use of radiopharmaceuticals is a powerful tool in the management of patients with infectious c inflammatory diseases in nuclear medicine. In this study ofloxacin as a second-generation fluoroquinolone is used to desig a desired infection imaging agent after labeling with 99mTc via direct labeling. Methods: Ofloxacin was radiolabeled with 99mTc using different concentrations of ligand, stannous chloride, sodiur pertechnetate and at different pH. Then labeling yield, stability in saline and serum, lipophilicity, binding wit Staphylococcus aureus and Escherichia coli and biodistribution in infected mice for labeled compound were studied. Results: The final complex, was characterized by TLC and HPLC and radiochemical purity of >90% was obtained when 1. mg ofloxacin in presence of 75 μg SnCl2 was labeled with 370 MBq sodium pertechnetate. The complex showed specifi binding to Staphylococcus aureus and Escherichia coli. Biodistribution results showed that radioligand had high affinity i the infected site in mice. The uptake for Staphylococcus aureus induced infections (TINT = 2.33 ± 0.17 at 1 h post injectior was higher than that was for Escherichia coli (TINT = 1.96 ± 0.13 at 1 h post injection). Conclusion: This complex may lead to further development of a radiotracer for imaging of infections induced by grair positive or gram-negative bacteria.
- Subjects
STAPHYLOCOCCUS aureus; ESCHERICHIA coli; DISEASE management; NUCLEAR medicine; FLUOROQUINOLONES; THIN layer chromatography; HIGH performance liquid chromatography
- Publication
Iranian Journal of Nuclear Medicine, 2013, Vol 21, Issue 1, p1
- ISSN
1681-2824
- Publication type
Article