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- Title
Genotypic characteristics of multidrug-resistant Escherichia coli isolates associated with urinary tract infections.
- Authors
Cao, Xiaoli; Zhang, Zhifeng; Shen, Han; Ning, Mingzhe; Chen, Junhao; Wei, Hongxia; Zhang, Kui
- Abstract
Escherichia coli is an important pathogen involved in community-acquired urinary tract infections ( CA- UTIs). In this study, we analyzed the prevalence of frequently occurring genes and the distribution of integrons in 51 multidrug-resistant ( MDR) E. coli isolates associated with CA- UTIs. The clonality of these strains was investigated by phylogrouping, multi-locus sequence typing, and pulsed-field gel electrophoresis ( PFGE). All these strains were found to produce two or more resistance determinants, ceftazidime-hydrolyzing CTX-M-type extended-spectrum β-lactamases ( ESBLs) and plasmid-mediated quinolone resistance determinants were the most prevalent (92.2% and 51.0%, respectively). A sulfhydryl variable-61-producing E. coli strain was identified for the first time in China. The prevalence of class 1 integrons was 54.9%, class 2 integrons were detected in three isolates but no isolate contained a class 3 integron. Phylogenetic group D was the dominant, observed in 70.6% of the isolates. PFGE analysis revealed a high level of diversity. Twenty-four distinctive sequence types ( STs) including four major STs ( ST648, ST224, ST38, and ST405) were identified. To our knowledge, this is the first report on the characterization of MDR E. coli isolates associated with CA- UTIs in China; our results suggest that an MDR D- ST648 clone producing CTX-M- ESBLs has emerged as a major clone in the community setting.
- Subjects
MULTIDRUG resistance; URINARY tract infections; ESCHERICHIA coli diseases; ESCHERICHIA coli; INTEGRONS
- Publication
APMIS, 2014, Vol 122, Issue 11, p1088
- ISSN
0903-4641
- Publication type
Article
- DOI
10.1111/apm.12260