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- Title
Effects of modified Ebbu Zhuyu Formula on pinopodes, microvilli, ERα and LIF expression of endometrium in aged mice.
- Authors
ZHOU Yumei; LIU Yanfeng; JINN Fanhui; CHEN Ran; ZHENG Hanyu; SHEN Mengmeng
- Abstract
Objective To observe the effects of modified Erbu Zhuyu (Double-tonifying Fertility-promoting) Formula on pinopodes, microvilli, estrogen receptor a (ERα) and leukemia inhibitory factor (LIF) expression of endometrium in aged mice. Methods Pregnant mice aged 8~9 months were randomly divided into aged model group, low-, mid- and high-dose modified Erbu Zhuyu Formula groups, progynova group and aspirin group (n = 8 in each group). Another 8 pregnant mice aged 6~8 weeks were selected as childbearing age blank group. The mice in childbearing age blank group and aged model group were perfused with 0.5% CMC solution every day, and the mice in the other groups were perfused with corresponding interventional medicines every day for 5 days. The expression of pinopodes and microvilli in endometrium was observed with scanning electron microscope. The protein localization of ERα and LIF in endometrium was evaluated with immunohistochemistry. The expression of ERα and LIF mRNA and protein in endometrium were also measured. Results Scanning electron microscope showed that mice in the childbearing age blank group presented with smooth endometrial surface, short and dense microvilli, and high-expressed pinopodes. In aged model group, the endometrial surface appeared uneven and seriously damaged, the microvilli were sparse, and pinopodes was almost invisible. The expression of micro villi and pinopodes in each treatment group was better than that in the aged model group. Compared with the childbearing age blank group, the expression of ERα mRNA and protein in the endometrium of aged model group increased (P < 0.05), and the expression of LIF mRNA and protein decreased (P < 0.05). Compared with aged model group, the expression of ERα mRNA and protein in endometrium of each treatment group decreased ( P <0. 05 ), and the expression of LIF mRNA and protein in endometrium of each treatment group increased (P <0.05) Conclusion Modified Erbu Zhuyu Formula can enrich the expression of microvilli and pinopodes, reduce the expression of ERα mRNA and protein, and increase the expression of LIF mRNA and protein, so as to improve endometrial receptivity.
- Subjects
GENE expression; LEUKEMIA inhibitory factor; ENDOMETRIUM; MICROVILLI; CHILDBEARING age
- Publication
Journal of Beijing University of Traditional Chinese Medicine, 2022, Issue 4, p398
- ISSN
1006-2157
- Publication type
Article
- DOI
10.3969/j.issn.1006-2157.2022.04.013