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- Title
Separation of two lipopolysaccharide populations with different contents of O-antigen factor 12<sub>2</sub> in <em>Salmonella enterica</em> serovar Typhimurium.
- Authors
Helander, Ilkka M.; Moran, Anthony P.; Mäkelä, P. Helena
- Abstract
Lipopolysaccharide (LPS) extraction from smooth-type Salmonella enterica sv. Typhimurium was carried out with the modified phenol/chloroform/petroleum ether method (volume ratio 5:5:8). In this procedure, LPS was precipitated from 90% phenol sequentially with water and acetone to yield LPS-H2O (minute amounts) and LPS-Ac (major amounts), respectively. Chemical analyses of the LPS fractions revealed that in the O antigen of LPS-H2O position C4 of the D-galactose was extensively glucosylated, corresponding to the 0-antigen factor 122. In LPS-Ac, this glucosylation was negligible. Inspection of the LPS fractions by sodium dodecyl sulphate/polyacrylamide gel electrophoresis and silver staining suggested that the glucosylation in LPS-H2O was pre-sent only in LPS species with a chain length higher than six repeating units.
- Subjects
ENDOTOXINS; MICROBIAL polysaccharides; BACTERIAL cell walls; SALMONELLA; ENTEROBACTERIACEAE; SALMONELLA typhimurium; PHENOLS
- Publication
Molecular Microbiology, 1992, Vol 6, Issue 19, p2857
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/j.1365-2958.1992.tb01465.x