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- Title
Discouraging cellular and bacterial adhesion on surfaces for bone temporary devices through ZrO2-Ag coatings.
- Authors
Cochis, Andrea; Ferraris, Sara; Scalia, Alessandro C.; Spriano, Silvia; Rimondini, Lia
- Abstract
INTRODUCTION: ideally bone temporary fixation devices must support the healing process but at the same time being surgically removed without hindering the newly formed tissue. Moreover, they should prevent bacterial infection preserving the healing site. Accordingly, here we propose a surface coating of biomedical Ti6Al4V surface with inorganic zirconia matrix embedding silver nanoclusters (ZrO2-Ag) where the Ag amount has been designed in order to prevent cells and bacteria adhesion avoiding cytotoxic side effect. EXPERIMENTAL: ZrO2-Ag coated specimens were produced introducing high (AH) or low (AL) amount of silver. Specimens were characterized for physical-chemical properties and then the biological assessment was performed by directly seeding human mesenchymal stem cells (hMSC) and the pathogen Staphylococcus aureus (S. aureus) to test the different surfaces in term of preventing adhesion. Finally, transcriptomics (RNAseq) and proteomics were applied to unravel the hMSC pathways regulated by the AH/AL-tuning. RESULTS AND DISCUSSION: AL and AH specimens reported a different release of silver over time (Fig. 1a). The biological characterization suggested AH being toxic for both hMSC and S. aureus that were unable to colonize the specimens due to a contact-killing effect. On the opposite, AL specimens reported very promising results: cells and bacteria were unable to colonize AL surfaces too, but they were viable thus suggesting for a non-toxic anti-adhesion effect. In fact, both transcriptomics (Fig. 1b) and proteomics (Fig. 1c) studies of non-adherent hMSC revealed a strong down-regulation of genes and proteins related with pro-adhesion processes, whereas pro-apoptotic pathways were not up-regulated. CONCLUSIONS: the AL ZrO2-Ag specimens reported a very promising solution for temporary fixation devices being able to prevent the surface colonization of both bacteria and cells by down-regulating the gene and proteins expression of several hMCS pro-adhesion pathways without cytotoxic effect.
- Subjects
BACTERIAL adhesion; BACTERIAL cell surfaces; HUMAN stem cells; MESENCHYMAL stem cells; TRANSCRIPTOMES; PROTEIN expression; ZIRCONIUM oxide
- Publication
Chemical Industry / Hemijska Industrija, 2024, Vol 78, p53
- ISSN
0367-598X
- Publication type
Article