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- Title
Mitogen-activated protein kinase 4-like carrying an MEY motif instead of a TXY motif is involved in ozone tolerance and regulation of stomatal closure in tobacco.
- Authors
Yuki Yanagawa; Hiroshi Yoda; Kohei Osaki; Yuta Amano; Mitsuko Aono; Shigemi Seo; Kazuyuki Kuchitsu; Ichiro Mitsuhara
- Abstract
The mitogen-activated protein kinases (MAPKs/MPKs) are important factors in the regulation of signal transduction in response to biotic and abiotic stresses. Previously, we characterized a MAPK from tobacco, Nicotiana tabacum MPK4 (NtMPK4). Here, we found a highly homologous gene, NtMPK4-like (NtMPK4L), in tobacco as well as other species in Solanaceae and Gramineae. Deduced amino acid sequences of their translation products carried MEY motifs instead of conserved TXY motifs of the MAPK family. We isolated the full length NtMPK4L gene and examined the physiological functions of NtMPK4L. We revealed that NtMPK4L was activated by wounding, like NtMPK4. However, a constitutively active salicylic acid-induced protein kinase kinase (SIPKKEE), which phosphorylates NtMPK4, did not phosphorylate NtMPK4L. Moreover, a tyrosine residue in the MEY motif was not involved in NtMPK4L activation. We also found that NtMPK4L-silenced plants showed rapid transpiration caused by remarkably open stomata. In addition, NtMPK4L-silenced plants completely lost the ability to close stomata upon ozone treatment and were highly sensitive to ozone, suggesting that this atypical MAPK plays a role in ozone tolerance through stomatal regulation.
- Subjects
TOBACCO research; MITOGEN-activated protein kinases; OZONE; GENETIC regulation in plants; CELLULAR signal transduction
- Publication
Journal of Experimental Botany, 2016, Vol 67, Issue 11, p3471
- ISSN
0022-0957
- Publication type
Article
- DOI
10.1093/jxb/erw173