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- Title
MOLECULAR BIOLOGICAL STUDIES OF THE ORIGIN OF BIFIDOBACTERIUM AND LACTOBACILLUS IN NEONATAL FECES.
- Authors
Rahman, Shafiqur; Nagata, Satoru; Matsuda, Kazunori; Tsuji, Hirokazu; Ogata, Kiyohito; Ozaki, Saori; Kawashima, Kazunari; Nomoto, Koji; Yamashiro, Yuichiro
- Abstract
We analyzed the composition of Bifidobacterium and Lactobacillus colonized in the human neonatal intestine and compared it with that in the mother. Forty-three healthy mothers and their neonates born by tram-vaginal delivery were enrolled in the study. We used reverse transcription -quantitative PCR targeting 16S rRNA to examine the population levels of Bifidobacterium and Lactobacillus in maternal feces (within 1 h after delivery), vaginal fluid (day 56 before delivery), breast milk (days 4 and 28 after delivery), and skin around the nipples (days 4 and 28 after delivery), and in neonatal meconium (within 1 h after birth) and feces (days 4 and 28 after birth). In the delivery period, Bifidobacterium and Lactobacillus were detected in the meconium at frequencies of 10% and 54%, respectively. Three out of the 4 species detected in the meconium were the same as those detected in the corresponding maternal feces and/or vaginal fluid. In contrast, detection rates of both Bifidobacterium and Lactobacillus in breast milk and on the skin around the nipples were far lower than in the maternal feces. From the results, it is expected that Bifidobacterium and Lactobacillus in neonates originate in the maternal feces and vaginal fluid.
- Subjects
MOLECULAR biology; BIFIDOBACTERIUM; LACTOBACILLUS; MICROBIOLOGY; FECES; NEWBORN infants; POLYMERASE chain reaction
- Publication
International Journal of Probiotics & Prebiotics, 2012, Vol 7, Issue 2, p91
- ISSN
1555-1431
- Publication type
Article