We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
The tumor immunity effect of 4-1BBL on human PBL-SCID OSCC chimeric model.
- Authors
SUN Shun-tao; YANG Hong-yu; LUO Juan; ZHU Guo-guang; LAI Ying -ming; YANG Hui-jun
- Abstract
PURPOSE: To establish OSSC hu-PBL-SCID chimeric model and evaluate the tumor immune effect of 4-1BBL on the mouse model. METHODS: Give the SCID mice 5x106 (0.2 mL) Tca8113 cells on the right back by subcutaneous injection. Injected anti-asialo-GML antibody from the tail vein after the tumor formed and divided into four groups randomly: Group 1 were not reconstructed tumor immune as a reference group. Group 2 were reconstructed tumor immune only. Group 3 were reconstructed tumor immune and injected adenovirus containing empty vector into the tumor. Group 4 were reconstructed tumor immune and injected adenovirus containing 4-1BBL into the tumor at the same time. Then, group 1 were injected 0.5mL 1640 medium into intraperitoneal, and group 2,3,4 were injected 3x107 (0.5 mL) lymphocytes of healthy volunteers at the same time. Recording the growth of the tumor weekly, and determined the humam IgG in the mice's blood at the second and the seventh week after the immune reconstruction by ELISA. Comparing the weight of the mice, the tumor and the spleen after the mice were killed. Determined the expression of 4-- 1BBL by PR-PCR, and determined the growth of the tumor and the spleen by HE. RESULTS: The tumorigenesity rate was 100% and the latency time was 12 to 18 days. At the second and seventh week after the immune reconstruction, the content of human IgG in Group 4 was 71.8µ/mL and 136.9µ/mL respectively, the growth of the tumor remarkably inhibited (P<0.01), and full expression of the 4-1BBL gene in the tumor was proved by RT-PCR, and pathological examination showed tumor growing and a large number of lymphocytes infiltrated the spleen. CONCLUSION: The hu-PBL-SCID OSCC chimeric model can be successfully established by combination of subcutaneous injection oral cancer cells and intraperitoneal injection human peripheral blood monocytes to the SCID mice. And the host's capacity of tumor inhibition can increase significantly after transfection.Supported by National Natural Science Foundation of China (30672335).
- Subjects
CHIMERISM; SUBCUTANEOUS infusions; IMMUNOGLOBULINS; ADENOVIRUSES; LYMPHOCYTES; POLYMERASE chain reaction; LABORATORY mice
- Publication
China Journal of Oral & Maxillofacial Surgery, 2012, Vol 10, Issue 5, p354
- ISSN
1672-3244
- Publication type
Article