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- Title
Effect of Influenza Virus Infection in a Murine Model of Asthma.
- Authors
Soo Kim, Hwan; Lee, Huisu; Sook Kim, Hyun; Won, Sulmui; Kyoung Lee, Eu; Bang, Kyongwon; Hong Chun, Yoon; Yoon, Jong-seo; Hee Kim, Hyun; Tack Kim, Jin; Kim, Hwan Soo; Kim, Hyun Sook; Lee, Eu Kyoung; Chun, Yoon Hong; Kim, Hyun Hee; Kim, Jin Tack
- Abstract
Respiratory virus infection is a major cause of asthma exacerbation. However, the underlying mechanisms of this exacerbation are unknown. Therefore, to determine the mechanisms, we examined the effect of influenza infection in a murine model of asthma. Mice were divided into four groups: the phosphate-buffered saline (PBS), house dust mite(HDM), influenza, and HDM/influenza groups. The influenza group and the HDM/influenza group were infected with influenza A virus. We measured airway resistance (Penh value), examined the lung tissue for pathology, and analyzed the cells and cytokines in bronchoalveolar lavage fluid (BALF) by ELISA. At 50 mg/mL methacholine, the HDM/influenza group showed a significantly higher Penh value than the PBS, HDM, and influenza groups. The number of neutrophils in BALF was higher in the HDM/influenza group than in the HDM group. A significantly greater number of lymphocytes and macrophages were detected in the HDM/influenza group than in the HDM group. IFN-γ and IL-1β levels were higher in the HDM/influenza group than in the HDM group. IL-5 levels did not vary between the HDM and HDM/influenza groups, IL-10 was significantly lower in the HDM/influenza than in the HDM group. Chemokine (C-X-C motif) ligand 1 (CXCL1) and regulated upon activation, normal T cell expressed and secreted (RANTES) were higher in the HDM/influenza group than in the HDM group. In a murine model of asthma, influenza-induced airway inflammation appeared to be caused by simultaneous activation of neutrophilic and eosinophilic inflammation.
- Subjects
AIRWAY (Anatomy); ANIMALS; ASTHMA; BIOLOGICAL models; CHEMOKINES; CYTOKINES; IMMUNITY; LUNGS; MICE; MITES; RESPIRATORY measurements; ORTHOMYXOVIRUS infections; DISEASE complications
- Publication
Iranian Journal of Allergy, Asthma & Immunology, 2015, Vol 14, Issue 4, p392
- ISSN
1735-1502
- Publication type
journal article