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- Title
Identification of Francisella tularensis Using Real-Time Fluorescence Polymerase Chain Reaction.
- Authors
McAvin, James C.; Morton, Marlan M.; Roudabush, Robert M.; Atchley, Daniel H.; Hickman, John R.
- Abstract
A Francisella tularensis-specific, TaqMan probe-based, real-time fluorescence polymerase chain reaction (PCR) assay required approximately 60 minutes and consistently achieved a sensitivity of ≤ 10 fg of F. tularensis genomic DNA (five genome equivalents). Specificity testing against a genomic DNA cross-reaction panel comprised of 22 bacterial organisms representing closely related species, diverse genera, and human genomic DNA resulted in no false positives of significance. The assay was conducted on a field-deployable thermocycler, the R.A.P.I.D. ("Ruggedized" Advanced Pathogen Identification Device), a microbial identification system that can provide rapid and accurate identification F. tularensis.
- Subjects
FLUORESCENCE; POLYMERASE chain reaction; DNA; GENOMES; CROSS reactions (Immunology)
- Publication
Military Medicine, 2004, Vol 169, Issue 4, p330
- ISSN
0026-4075
- Publication type
Article
- DOI
10.7205/MILMED.169.4.330