We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Regeneration of critical-sized mandibular defect using a 3D-printed hydroxyapatite-based scaffold: An exploratory study.
- Authors
Chang, Po‐Chun; Luo, Hui‐Ting; Lin, Zhi‐Jie; Tai, Wei‐Chiu; Chang, Ching‐He; Chang, Ying‐Chieh; Cochran, David L.; Chen, Min‐Huey; Chang, Po-Chun; Luo, Hui-Ting; Lin, Zhi-Jie; Tai, Wei-Chiu; Chang, Ching-He; Chang, Ying-Chieh; Chen, Min-Huey
- Abstract
<bold>Background: </bold>Three-dimensional (3D) printing has become an available technology to fabricate customized tissue engineering scaffolds with delicate architecture. This exploratory study aimed to evaluate the potential of a 3D-printed hydroxyapatite-based scaffold as a biomaterial for obtaining guided bone regeneration (GBR) in vivo.<bold>Methods: </bold>Scaffolds composed of 90% hydroxyapatite and 10% poly(lactic-co-glycolic acid) were printed using a microextrusion process to fit 4 mm diameter and 0.5 mm thick through-and-through osseous defects on the mandibular ramus of rats, with unfilled defects serving as controls. Specimens were analyzed for regeneration-associated gene expression on day 7, and micro-computed tomography (micro-CT) and histology assessments were carried out on day 28.<bold>Results: </bold>The scaffolds were 3.56 ± 0.43 mm (x-axis) and 4.02 ± 0.44 mm (y-axis) in diameter and 0.542 ± 0.035 mm thick (z-axis), with a mean pore size of 0.420 ± 0.028 × 0.328 ± 0.005 mm2 . Most scaffolds fit the defects well. Type I collagen, VEGF, and Cbfa1 were upregulated in the scaffold-treated defects by day 7. By day 28, de novo osteogenesis and scaffold-tissue integration were evident in the scaffold-treated defects, and entire mineralized tissue, as well as newly formed bone, was significantly promoted, as seen in the micro-CT and histologic analyses.<bold>Conclusion: </bold>The 3D-printed hydroxyapatite-based scaffold showed acceptable dimensional stability and demonstrated favorable osteoregenerative capability that fulfilled the need for GBR.
- Subjects
GUIDED bone regeneration; TISSUE scaffolds; MANDIBLE abnormalities; THREE-dimensional printing; HYDROXYAPATITE; RESEARCH; BONE growth; ANIMAL experimentation; CULTURE media (Biology); RESEARCH methodology; MEDICAL cooperation; EVALUATION research; RATS; COMPARATIVE studies; MINERALS; BONE regeneration; COMPUTED tomography
- Publication
Journal of Periodontology, 2021, Vol 92, Issue 3, p428
- ISSN
0022-3492
- Publication type
journal article
- DOI
10.1002/JPER.20-0110