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- Title
Characterization of oral immune cells in birch pollen-allergic patients: impact of the oral allergy syndrome and sublingual allergen immunotherapy on antigen-presenting cells.
- Authors
Mascarell, L.; Rak, S.; Worm, M.; Melac, M.; Soulie, S.; Lescaille, G.; Lemoine, F.; Jospin, F.; Paul, S.; Caplier, L.; Hasséus, B.; Björhn, C.; Zeldin, R. K.; Baron‐Bodo, V.; Moingeon, P.
- Abstract
Background: A detailed characterization of human oral immune cells is needed to better understand local mechanisms associated with allergen capture following oral exposure. Methods: Oral immune cells were characterized by immunohistology and immunofluorescence in biopsies obtained from three healthy individuals and 23 birch pollen-allergic patients with/without oral allergy syndrome (OAS), at baseline and after 5 months of sublingual allergen immunotherapy (AIT). Results: Similar cell subsets (i.e., dendritic cells, mast cells, and T lymphocytes) were detected in oral tissues from healthy and birch pollen-allergic individuals. CD207+ Langerhans cells (LCs) and CD11c+ myeloid dendritic cells (DCs) were found in both the epithelium and the papillary layer of the Lamina propria (LP), whereas CD68+ macrophages, CD117+ mast cells, and CD4+/CD8+ T cells were rather located in both the papillary and reticular layers of the LP. Patterns of oral immune cells were identical in patients with/without OAS, except lower numbers of CD207+ LCs found in oral tissues from patients with OAS, when compared to OAS- patients (P < 0.05). A 5-month sublingual AIT had a limited impact on oral immune cells, with only a significant increase in IgE+ cells in patients from the active group. Colocalization experiments confirmed that such IgE-expressing cells mostly encompass CD68+ macrophages located in the LP, and to a lesser extent CD207+ LCs in the epithelium. Conclusion: Two cell subsets contribute to antigen/allergen uptake in human oral tissues, including (i) CD207+ LCs possibly involved in the physiopathology of OAS and (ii) CD68+ macrophages likely critical in allergen capture via IgE-facilitated mechanisms during sublingual AIT.
- Subjects
IMMUNOTHERAPY; ALLERGENS; ALLERGIC rhinitis; IMMUNOFLUORESCENCE; LANGERHANS cells; EPITHELIUM; MAST cells
- Publication
Allergy, 2015, Vol 70, Issue 4, p408
- ISSN
0105-4538
- Publication type
Article
- DOI
10.1111/all.12576