We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Magnetic hyperthermia enhance the treatment efficacy of peri-implant osteomyelitis.
- Authors
Chih-Hsiang Fang; Pei-I Tsai; Shu-Wei Huang; Jui-Sheng Sun; Jenny Zwei-Chieng Chang; Hsin-Hsin Shen; San-Yuan Chen; Feng Huei Lin; Lih-Tao Hsu; Yen-Chun Chen; Fang, Chih-Hsiang; Tsai, Pei-I; Huang, Shu-Wei; Sun, Jui-Sheng; Chang, Jenny Zwei-Chieng; Shen, Hsin-Hsin; Chen, San-Yuan; Lin, Feng Huei; Hsu, Lih-Tao; Chen, Yen-Chun
- Abstract
<bold>Background: </bold>When bacteria colony persist within a biofilm, suitable drugs are not yet available for the eradication of biofilm-producing bacteria. The aim of this study is to study the effect of magnetic nano-particles-induced hyperthermia on destroying biofilm and promoting bactericidal effects of antibiotics in the treatment of osteomyelitis.<bold>Methods: </bold>Sixty 12-weeks-old male Wistar rats were used. A metallic 18G needle was implanted into the bone marrow cavity of distal femur after the injection of Methicillin-sensitive Staphylococcus aureus (MSSA). All animals were divided into 5 different treatment modalities. The microbiological evaluation, scanning electron microscope examination, radiographic examination and then micro-CT evaluation of peri-implant bone resorption were analyzed.<bold>Results: </bold>The pathomorphological characteristics of biofilm formation were completed after 40-days induction of osteomyelitis. The inserted implants can be heated upto 75 °C by magnetic heating without any significant thermal damage on the surrounding tissue. We also demonstrated that systemic administration of vancomycin [VC (i.m.)] could not eradicate the bacteria; but, local administration of vancomycin into the femoral canal and the presence of magnetic nanoparticles hyperthermia did enhance the eradication of bacteria in a biofilm-based colony. In these two groups, the percent bone volume (BV/TV: %) was significantly higher than that of the positive control.<bold>Conclusions: </bold>For the treatment of chronic osteomyelitis, we developed a new modality to improve antibiotic efficacy; the protection effect of biofilms on bacteria could be destroyed by magnetic nanoparticles-induced hyperthermia and therapeutic effect of systemic antibiotics could be enhanced.
- Subjects
MAGNETIC nanoparticle hyperthermia; OSTEOMYELITIS; METHICILLIN-resistant staphylococcus aureus; SCANNING electron microscopes; VANCOMYCIN; INFECTION treatment; OSTEOMYELITIS treatment; STAPHYLOCOCCAL disease treatment; COMPLICATIONS of prosthesis; ANIMAL experimentation; ANTIBIOTICS; BIOFILMS; IRON compounds; RATS; STAPHYLOCOCCAL diseases; THERMOTHERAPY; TREATMENT effectiveness; PHARMACODYNAMICS; EQUIPMENT &; supplies; THERAPEUTICS
- Publication
BMC Infectious Diseases, 2017, Vol 17, p1
- ISSN
1471-2334
- Publication type
journal article
- DOI
10.1186/s12879-017-2621-4