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- Title
Regulation of voltage-gated K<sup>+</sup> channels by glucose metabolism in pancreatic β-cells
- Authors
Yoshida, Masashi; Dezaki, Katsuya; Yamato, Shiho; Aoki, Atsushi; Sugawara, Hitoshi; Toyoshima, Hideo; Ishikawa, San-e; Kawakami, Masanobu; Nakata, Masanori; Yada, Toshihiko; Kakei, Masafumi
- Abstract
Abstract: Regulation of delayed rectifier-type K+ channels (Kv-channels) by glucose was studied in rat pancreatic β-cells. The Kv-channel current was increased in amplitudes by increasing glucose concentration from 2.8 to 16.6mM, while it was decreased by 2.8mM glucose in a reversible manner (down-regulation) in both perforated and conventional whole-cell modes. The current was decreased by FCCP, intrapipette 0mM ATP or AMPPNP. Glyceraldehyde, pyruvic acid, 2-ketoisocaproic acid, and 10mM MgATP prevented the down-regulation induced by 2.8mM or less glucose. The residual current after treatment with Kv2.1-specific blocker, guangxitoxin-1E, was unchanged by lowering or increasing glucose concentration. We conclude that glucose metabolism regulates Kv2.1 channels in rats β-cells via altering MgATP levels.
- Subjects
CARBOHYDRATE metabolism; GLUCOSE; POTASSIUM channels; PANCREATIC beta cells; SECRETION; INSULIN; LABORATORY rats
- Publication
FEBS Letters, 2009, Vol 583, Issue 13, p2225
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2009.05.050