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- Title
Sustained activation of AMP-activated protein kinase induces c-Jun N-terminal kinase activation and apoptosis in liver cells
- Authors
Meisse, Delphine; Van de Casteele, Mark; Beauloye, Christophe; Hainault, Isabelle; Kefas, Benjamin A.; Rider, Mark H.; Foufelle, Fabienne; Hue, Louis
- Abstract
The aim of this work was to study the effect of a sustained activation of AMP-activated protein kinase (AMPK) on liver cell survival. AMPK activation was achieved by incubating FTO2B cells with AICA-riboside, which is transformed into ZMP, an AMP analogue, or by adenoviral transfection of hepatocytes with a constitutively active form of AMPK. Prolonged AMPK activation triggered apoptosis and activated c-Jun N-terminal kinase (JNK) and caspase-3. Experiments with iodotubercidin, dicoumarol and z-VAD-fmk, which inhibited AMPK, JNK and caspase activation, respectively, supported the notion that prolonged AMPK activation in liver cells induces apoptosis through an activation pathway that involves JNK and caspase-3.
- Subjects
PROTEIN kinases; LIVER cells
- Publication
FEBS Letters, 2002, Vol 526, Issue 1-3, p38
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)03110-1