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- Title
Screening of cannabinoids in industrial-grade hemp using two-dimensional liquid chromatography coupled with acidic potassium permanganate chemiluminescence detection.
- Authors
Pandohee, Jessica; Holland, Brendan J.; Li, Bingshan; Tsuzuki, Takuya; Stevenson, Paul G.; Barnett, Neil W.; Pearson, James R.; Jones, Oliver A.H.; Conlan, Xavier A.
- Abstract
Widely known for its recreational use, the cannabis plant also has the potential to act as an antibacterial agent in the medicinal field. The analysis of cannabis plants/products in both pharmacological and forensic studies often requires the separation of compounds of interest and/or accurate identification of the whole cannabinoid profile. In order to provide a complete separation and detection of cannabinoids, a new two-dimensional liquid chromatography method has been developed using acidic potassium permanganate chemiluminescence detection, which has been shown to be selective for cannabinoids. This was carried out using a Luna 100 Å CN column and a Poroshell 120 EC-C18 column in the first and second dimensions, respectively. The method has utilized a large amount of the available separation space with a spreading angle of 48.4° and a correlation of 0.66 allowing the determination of more than 120 constituents and mass spectral identification of ten cannabinoids in a single analytical run. The method has the potential to improve research involved in the characterization of sensitive, complex matrices.
- Subjects
CANNABIS (Genus); MEDICINAL plants; ANTIBACTERIAL agents; SEPARATION (Technology); CANNABINOIDS; LIQUID chromatography; CHEMILUMINESCENCE
- Publication
Journal of Separation Science, 2015, Vol 38, Issue 12, p2024
- ISSN
1615-9306
- Publication type
Article
- DOI
10.1002/jssc.201500088