We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Comparative transcriptome analysis of Saposhnikovia divaricata to reveal drought and rehydration adaption strategies.
- Authors
Su, Youla; Jiao, Miaomiao; Guan, Huan; Zhao, Yuhuan; Deji, Cuomu; Chen, Guilin
- Abstract
Background: Water scarcity has become one of the most prevalent environmental factors adversely affecting plant growth and development. Different species have developed multiple ways of drought resistance. Saposhnikovia divaricata is a commonly used traditional herb in East Asia. However, limited information is available on the drought response of this herb and further clarification of underlying molecular mechanism remains a challenge. Methods and results: In this study, a comparative transcriptome analysis was firstly conducted to identify the major pathways and candidate genes involved in the drought adaptive response of S. divaricata. The seedlings of S. divaricata were subjected to progressive drought by withholding water for 16 days followed by 8 days of rehydration. Transcriptome analysis identified a total of 89,784 annotated unigenes. The number of differentially expressed genes (DEGs) gradually increased with the deepening of drought and decreased after rehydration. Gene Ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis suggested genes related to oxidoreductase activity, carbohydrate metabolism, plant hormone signaling pathway and secondary metabolism were important in drought response of S. divaricata. Specific genes involved in reactive oxygen species scavenging system (POD, Cu/Zn-SOD, APX), abscisic acid and jasmonic acid signaling pathway (PYL4, PP2Cs, JAR1, JAZ) and phenylpropanoid biosynthesis (4CL, CCR, CAD) underwent dynamic alterations under drought and rehydration. Finally, the expression pattern of 12 selected DEGs from the transcriptomic profiling was validated by real-time quantitative PCR. Conclusion: Our study laid a foundation for understanding the stress response of S. divaricata and other Apiaceae family plant at molecular level.
- Subjects
EAST Asia; DROUGHT tolerance; DROUGHTS; WATER shortages; JASMONIC acid; REACTIVE oxygen species; SECONDARY metabolism; CARROTS
- Publication
Molecular Biology Reports, 2023, Vol 50, Issue 4, p3493
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-023-08305-8