We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Cyelins and Cyelin Dependent Kinases during Cardiac Development.
- Authors
Min Jeong Kang; Jin-Sang Kim; Soo-Wan Chae; Keum Nim Koh; Gou Young Koh
- Abstract
The molecular mechanisms that regulate the cardiomyocyte cell cyele and its terminal differentiation remain largely unknown. To determine which cyelins or cyelin dependent kinases (CDKs) are important for cardiomyocyte proliferation, we examined the expression of cyelins and CDKs during normal cardiac development. All cyelins and CDKs were highly expressed during embryonic cardiac development, then they decreased at different rates after birth. The mRNAs and proteins of cyelins A and B (G2 and M phase cyelins) were found in embryonic and neonatal hearts, but were not detected in young or adult hearts. In contrast, while the mRNAs of cyelins Dl, D2, D3, and E (Gl and S phase cyelins) were observed during all stages of development, the proteins of cyelins Dl, D3, and E were observed in hearts at the young growth stage, although the levels decreased differently. Reverse transcriptase-polymerase chain reaction (RT-PCR) using specific cyelin Band D3 primers revealed that cyelins Band D3 originated from cardiomyocytes and noncardiomyocytes. The CDKs (cdc2, CDK2, and CDK4) were highly expressed during embryonic cardiac development and maintained almost constant levels during neonatal periods. However, they were expressed at very low levels at the young and adult stages. The pattern of proliferating cell nuelear antigen (PCNA) expression during cardiac development was similar to the expression of CDKs. These findings suggest that all cyelins and CDKs are involved in the cardiac cell cyele, and that marked and rapid reduction of mitotic cyelins may be associated with the withdrawal of the cardiac cell cyele after birth.
- Publication
Molecules & Cells (Springer Nature), 1997, Vol 7, Issue 3, p360
- ISSN
1016-8478
- Publication type
Article
- DOI
10.1016/s1016-8478(23)13306-1