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- Title
De novo biosynthesis of C-arabinosylated flavones by utilization of indica rice C-glycosyltransferases.
- Authors
Chen, Zhuo; Sun, Yuwei; Wang, Guangyi; Zhang, Ying; Zhang, Qian; Zhang, Yulian; Li, Jianhua; Wang, Yong
- Abstract
Flavone C-arabinosides/xylosides are plant-originated glycoconjugates with various bioactivities. However, the potential utility of these molecules is hindered by their low abundance in nature. Engineering biosynthesis pathway in heterologous bacterial chassis provides a sustainable source of these C-glycosides. We previously reported bifunctional C-glucosyl/C-arabinosyltransferases in Oryza sativa japonica and O. sativa indica, which influence the C-glycoside spectrum in different rice varieties. In this study, we proved the C-arabinosyl-transferring activity of rice C-glycosyltransferases (CGTs) on the mono-C-glucoside substrate nothofagin, followed by taking advantage of specific CGTs and introducing heterologous UDP-pentose supply, to realize the production of eight different C-arabinosides/xylosides in recombinant E. coli. Fed-batch fermentation and precursor supplement maximized the titer of rice-originated C-arabinosides to 20–110 mg/L in an E. coli chassis. The optimized final titer of schaftoside and apigenin di-C-arabinoside reached 19.87 and 113.16 mg/L, respectively. We demonstrate here the success of de novo bio-production of C-arabinosylated and C-xylosylated flavones by heterologous pathway reconstitution. These results lay a foundation for further optimal manufacture of complex flavonoid compounds in microbial cell factories.
- Subjects
BIOSYNTHESIS; RICE; MICROBIAL cells; COMPLEX compounds; APIGENIN; FLAVONES; FLAVONOIDS; GLYCOSIDES
- Publication
Bioresources & Bioprocessing, 2021, Vol 8, Issue 1, p1
- ISSN
2197-4365
- Publication type
Article
- DOI
10.1186/s40643-021-00404-3