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- Title
Alkalisalibacterium limincola gen. nov., sp. nov., an Alkaliphilic Bacterium Isolated from an Alkaline, Saline Lake of Buryatia in Russia.
- Authors
Abidueva, E. Y.; Kudryashova, E. B.; Ariskina, E. V.; Liu, Sh.-W.; Sun, Ch.-H.; Karlyshev, A. V.
- Abstract
A gram-stain-negative, aerobic, rod-shaped and motile bacterium, designated Alg18-2.2T, was isolated from a bottom sediment of the highly alkaline-saline lake in Buryatia, Russia. The isolate is facultatively alkaliphilic, growing at pH 7.0–12.0 (optimum at 9.0–9.5), and salt-tolerant up to 7% NaCl (w/v; optimum, 1%, w/v). Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain Alg18-2.2T formed a distinct monophyletic lineage within the family Lysobacteraceae. Strain Alg18-2.2T had highest sequence similarity to Lysobacter concretionis Ko07T (95.0%), Lysobacter maris KMU-14T (95.0%), Coralloluteibacterium stylophorae Sty a-1T (94.9%) and Lysobacter psychrotolerans ZS60T (94.8%). The ANI values between Alg18-2.2T and representatives of genera within order Lysobacterales were 66.4–76.5%, which is below of the statistical threshold for the delineation of the species. The AAI values between the strain and representatives of genera within order Lysobacterales were 52.2–66.4%, which is consistent with the data intra-order AAI values for Lysobacterales. The quinone system consisted of ubiquinone 8 (Q-8). Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids were presented as major lipids in the polar lipid profile. The major cellular fatty acids were 9-Methyl C16:0 (23.9%), iso-C15:0 (22.4%), iso-C16:0 (16.3%) and iso-C17:0 (14.6%). The genome has a G+C content of 68.7 mol % and a total assembly size of 3,025,752 bases. Based on polyphasic results including phylogenomic data, we propose a new genus and species Alkalisalibacterium limincola gen. nov., sp. nov. with the type strain Alg18-2.2T (=CPCC 101300Т = DSM 110880T = VKM B-3394T).
- Subjects
BURYATIA (Russia); RUSSIA; SALT lakes; PHOSPHATIDYLGLYCEROL; BACTERIA; PHOSPHOLIPIDS; FATTY acids; UBIQUINONES; EFFECT of salt on plants
- Publication
Microbiology (00262617), 2023, Vol 92, Issue 1, p11
- ISSN
0026-2617
- Publication type
Article
- DOI
10.1134/S0026261722602548