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- Title
Prevalence and specificity of the enhancing effect of three types of interleukin 2 on T cell responsiveness in 97 lepromatous leprosy patients of mixed ethnic origin.
- Authors
Barnass, S.; Mace, J.; Steele, J.; Torres, P.; Gervasonl, B.; Ravioli, R.; Ternncio, J.; Rook, G. A. W.; Waters, M. F. R.
- Abstract
Peripheral blood mononuclear cells from 97 predominantly lepromatous leprosy patients and 11I control subjects were tested in a lymphoproliferative assay for response to Mycobacterium leprae (whole and sonicated), and sonicated M. vaccae, M. tuberculosis, and M. serofulaceum, in the presence and absence of three types of interlcukin 2 (IL-2) (crude, purified, and recombinant). IL-2 enhanced the response to sonicated M, tuberculosis and M. leprae organisms more often in patients than in control subjects, but not significantly so and only in a minority of patients. This effect was significantly more common (though still only found in a minority of 46%) using M. leprae organisms as antigen, than when using sonicates of M. leprae (19%) or M. raccae (19%). However it was nearly as frequent using sonicated M. tuberculosis, or M. scrofulaceum. Thus in only nine patients was the effect specific to M. leprae. Enhancement by IL-2 could not be related to the type of IL-2 used, the dose of antigen, or the amount of endogenous IL-2 released by the cells tested. Similarly it was not related to the extent to which IL-2 caused increased background proliferation in control wells, which occurred to an equal extent using cells from control subjects, nor was it related to the extent of antigen-driven proliferation. The data have also been analysed in relation to duration of disease (50 years to a few weeks) and ethnic origin. No correlations have been revealed. Thus enhancement by IL-2 of the lymphoproliferative response to mycobacterial antigens does occur using cells from lepromatous leprosy patients, but it is found in a minority of patients, it is not specific to M. leprae, and can occur with cells from normal donors.
- Subjects
INTERLEUKIN-2; TUBERCULOSIS; BLOOD; ANTIGENS; IMMUNOGLOBULINS; MYCOBACTERIAL diseases
- Publication
Clinical & Experimental Immunology, 1986, Vol 64, Issue 1, p41
- ISSN
0009-9104
- Publication type
Article