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- Title
Efecto de los crioprotectores en la morfología y pérdida iónica en yemas axilares de vid cv. 'Flame Seedless' crioconservadas.
- Authors
Lazo-Javalera, María Fernanda; Astorga-Cienfuegos, Karen Rosalinda; Tiznado-Hernández, Martín Ernesto; Vargas-Arispuro, Irasema; Martínez-Télle, MiguelÁngel; Islas-Osuna, María Auxiliadora; Hernández-Oñate, Miguel Ángel; Martínez-Montero, Marcos Edel; Rivera-Domínguez, Marisela
- Abstract
The cryopreservation has revolutionized the field of biotechnology. Frozen in liquid nitrogen (LN) preserves long living cells. In this sense, in this paper were evaluated three conditions of cryopreservation based on vitrification of buds of grapevine. The buds were subjected to the PVS2, PVS3 and glycerol for 0-420 min, and submerged in LN for one hour. After the incubation time electrolyte leakage was determined as a viability measurement, and tissue damage was evaluated through stereoscopic observation. Based on the viability percentage the best preservation method was using PVS3 solution (30%) followed by glycerol (25%) and PVS2 (<10%). The images of the buds exposed to PVS3 shows no tissue damage unlike to PVS2 and glycerol, were not sufficient to preserve buds tissue. The results shown here suggest that using PVS3 as protocol can be considered for buds grapevine germplasm preservation
- Publication
Investigación y Ciencia de la Universidad Autónoma de Aguascalientes, 2017, Vol 25, Issue 72, p36
- ISSN
1665-4412
- Publication type
Article
- DOI
10.33064/iycuaa201772220