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- Title
SEROLOGICAL AND MOLECULAR SURVEY OF TICK-BORNE PATHOGENS AMONG DOGS IN NORTHERN BRAZIL.
- Authors
dos Anjos Pacheco, Thábata; Oliveira Maia, Maerle; Witter, Rute; Meneguzzi, Mirella; Tomé Melo, Andréia Lima; Nakazato, Luciano; Dutra, Valéria; Moura de Aguiar, Daniel; de Campos Pacheco, Richard
- Abstract
This study aimed to evaluate the occurrence of anti-Rickettsia spp. and anti-Ehrlichia canis antibodies, as well as the molecular detection of Babesia vogeli, Ehrlichia spp. and Hepatozoon spp. DNA in dogs from Ji-Paraná municipality in the state of Rondônia, northern Brazil. For this purpose, we analyzed 177 serum and blood samples using indirect immunofluorescence assay (IFA) and polymerase chain reaction (PCR), respectively. A total of 8.47% (15/177) of dogs were seroreactive to at least one Rickettsia species, with endpoint titers ranging from 64 to 1,024. The highest endpoint titers were observed for Rickettsia parkeri and Rickettsia rhipicephali. Furthermore, anti-E. canis antibodies were detected in 24.29% (43/177) of the tested serum samples, with endpoint titers ranging from 80 to 2,560. Among the blood samples analyzed molecularly, 5.65% (10/177), 22.03% (39/177), and 48.02% (85/177) were infected with B. vogeli, E. canis, and Hepatozoon canis, respectively. Herein, we provide serological evidence of Rickettsia spp. and E. canis infection, and confirmed the occurrence of B. vogeli, E. canis, and H. canis DNA in dogs from the rural area of the state of Rondônia, in the Western Amazon.
- Subjects
RONDONIA (Brazil : State); BRAZIL; RICKETTSIA; DOGS; CANIS; POLYMERASE chain reaction
- Publication
Archives of Veterinary Science, 2021, Vol 26, Issue 4, p31
- ISSN
1517-784X
- Publication type
Article