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- Title
Antimicrobial-Resistant and Extraintestinal Pathogenic Escherichia coli in Retail Foods.
- Authors
Johnson, James R.; Kuskowski, Michael A.; Smith, Kirk; O'Bryan, Timothy T.; Tatini, Sita
- Abstract
Background. Extraintestinal Escherichia coli infections are associated with specialized extraintestinal pathogenic E. coli (ExPEC) strains and, increasingly, with antimicrobial resistance. The food supply may disseminate ExPEC and antimicrobial-resistant E. coli. Methods. In a prospective survey of 1648 diverse food items from 10 retail markets in the MinneapolisSt. Paul area during 2001–2003, selective cultures and disk-diffusion assays for the isolation and characterization of antimicrobial-resistant E. coli and polymerase chain reaction-based assays and O serotyping to define ExPEC-associated traits were performed. Results. E. coli contamination exhibited a prevalence gradient from miscellaneous foods (9%), through beef or pork (69%), to poultry (92%; P<.001). Among E. coli-positive samples, similar prevalence gradients were detected for antimicrobial resistance (27%, 85%, and 94% of samples, respectively; P<.001) and ExPEC contamination (4%, 19%, and 46%, respectively; P<.001). By multivariate analysis, beef or pork and poultry from natural-food stores exhibited reduced risks of E. coli contamination and antimicrobial resistance. Indirect evidence suggested on-farm selection of resistance. Four food-source ExPEC isolates (from pea pods, turkey parts, ground pork, and vegetable dip) closely resembled selected human clinical isolates by O antigen and genomic profile. Conclusions. Retail foods may be an important vehicle for community-wide dissemination of antimicrobialresistant E. coli and ExPEC, which may represent a newly recognized group of medically significant foodborne pathogens.
- Subjects
ESCHERICHIA coli diseases; ESCHERICHIA coli; DRUG resistance in microorganisms; FOOD; POLYMERASE chain reaction; PATHOGENIC microorganisms
- Publication
Journal of Infectious Diseases, 2005, Vol 191, Issue 7, p1040
- ISSN
0022-1899
- Publication type
Article
- DOI
10.1086/428451