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- Title
Effect of bronchial allergen challenge on in vitro cytokine release by peripheral blood mononuclear cells of atopic patients.
- Authors
Abbal, C.; Persi, L.; Bousquet, J.; Yssel, H.; Pène, J.
- Abstract
Background During the pollen season, peripheral blood mononuclear cells (PBMC) from allergic patients produce increased levels of Th2 cytokines after stimulation with allergen <em>in vitro</em>. We have studied the effect of a single bronchial provocation test (BPT) of allergic patients to determine whether allergen challenge <em>in vivo</em> modulates cytokine production by PBMC, after subsequent stimulation with the same allergen <em>in vitro</em>. Methods Twelve atopic asthmatic patients were challenged with the relevant allergen, and their PBMC, isolated before (T0) or 6 (T6) or 24 h (T24) after BPT, respectively, were cultured for 120 h in the presence or absence of the same allergen, after which cytokine production was measured by ELISA. Results Allergen-specific activation of the PBMC at T0 resulted in interleukin (IL)-5 and IL-13 production, but not in detectable levels of interferon-gamma and IL-4. BPT did not induce the secretion of the latter cytokines. However, IL-5 and lL-13 production was significantly decreased at T24, as compared to T0. No statistically significant differences were found between the production of IL-10 before and after BPT. Conclusions In contrast to the effects of natural challenge with allergen, a decrease in the production of some Th2 cytokines by peripheral blood T cells was observed 24 h after BPT, suggesting a concomitant decrease in the frequency of allergen-specific T cells in the circulation.
- Subjects
ALLERGENS; CYTOKINES; BLOOD cells; ATOPIC dermatitis; ANTIGENS
- Publication
Allergy, 1998, Vol 53, Issue 10, p945
- ISSN
0105-4538
- Publication type
Article
- DOI
10.1111/j.1398-9995.1998.tb03794.x