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- Title
The transcriptional landscape of the developmental switch from regular pollen maturation towards microspore-derived plant regeneration in barley.
- Authors
Nowicka, Anna; Kovacik, Martin; Maksylewicz, Anna; Kopeć, Przemysław; Dubas, Ewa; Krzewska, Monika; Springer, Agnieszka; Hoffie, Robert E.; Daghma, Diaaeldin S.; Milec, Zbyněk; Pecinka, Ales; Kumlehn, Jochen; Żur, Iwona
- Abstract
Plant formation from in vitro-cultivated microspores involves a complex network of internal and environmental factors. Haploids/doubled haploids (DHs) derived from in vitro-cultured microspores are widely used in plant breeding and genetic engineering. However, the mechanism underlying the developmental switch from regular pollen maturation towards microspore-derived plant regeneration remains poorly defined. Here, RNA-sequencing was employed to elucidate the transcriptional landscapes of four early stages of microspore embryogenesis (ME) in barley cultivars Golden Promise and Igri, which exhibit contrasting responsiveness to microspore-derived plant formation. Our experiments revealed fundamental regulatory networks, specific groups of genes, and transcription factor (TF) families potentially regulating the developmental switch. We identified a set of candidate genes crucial for genotype-dependent responsiveness/recalcitrance to ME. Our high-resolution temporal transcriptome atlas provides an important resource for future functional studies on the genetic control of microspore developmental transition.
- Subjects
REGENERATION (Botany); PLANT breeding; TRANSCRIPTION factors; GENETIC engineering; POLLEN
- Publication
Crop Journal (2095-5421), 2024, Vol 12, Issue 4, p1064
- ISSN
2095-5421
- Publication type
Article
- DOI
10.1016/j.cj.2024.07.003