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- Title
Royal jelly fatty acids downregulate ANGPTL8 expression through the decrease in HNF4α protein in human hepatoma HepG2 cells.
- Authors
Yuki Inoue; Marina Ienaga; Tetsuro Kamiya; Tetsuo Adachi; Mitsuhiro Ohta; Hirokazu Hara
- Abstract
Royal jelly (RJ) intake has been reported to be effective for reducing serum lipids; however, the mechanism is not fully understood. Angiopoietin-like protein 8 (ANGPTL8), a secreted protein, plays a key role in lipid metabolism. In this study, we investigated the effects of specific fatty acids included in RJ (RJ fatty acids), such as 10-hydroxy-2-decenoic acid, 10-hydroxydecanoic acid, and sebacic acid (SA), on expression of ANGPTL8 in human hepatoma HepG2 cells. SA markedly reduced the expression of ANGPTL8. Reporter assay revealed that SA suppressed ANGPTL8 promoter activity. In addition, we identified a functional binding site of hepatocyte nuclear factor-4γ(HNF4α), a liver-enriched transcription factor, in the ANGPTL8 promoter. SA reduced the levels of HNF4γprotein and the binding of HNF4γto the ANGPTL8 promoter. Moreover, siRNA knockdown of HNF4γsuppressed the expression of ANGTPL8 mRNA. Taken together, we conclude that SA downregulates ANGPTL8 expression via the decrease in HNF4γprotein.
- Subjects
ROYAL jelly; FATTY acids; ANGIOPOIETIN-like proteins; HEPATOCELLULAR carcinoma; BLOOD lipids; TRANSCRIPTION factors
- Publication
Bioscience, Biotechnology & Biochemistry, 2022, Vol 86, Issue 6, p747
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1093/bbb/zbac043