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- Title
Validation of a screening method for the simultaneous identification of fat-soluble and water-soluble vitamins (A, E, B<sub>1</sub>, B<sub>2</sub> and B<sub>6</sub>) in an aqueous micellar medium of hexadecyltrimethylammonium chloride.
- Authors
León-Ruiz, V.; Vera, S.; San Andrés, M. P.
- Abstract
Simultaneous determination of the fat-soluble vitamins A and E and the water-soluble vitamins B1, B2 and B6 has been carried using a screening method from fluorescence contour graphs. These graphs show different colour zones in relation to the fluorescence intensity measured for the pair of excitation/emission wavelengths. The identification of the corresponding excitation/emission wavelength zones allows the detection of different vitamins in an aqueous medium regardless of the fat or water solubility of each vitamin, owing to the presence of a surfactant which forms micelles in water at the used concentration (over the critical micelle concentration). The micelles dissolve very water insoluble compounds, such as fat-soluble vitamins, inside the aggregates. This approach avoids the use of organic solvents in determining these vitamins and offers the possibility of analysing fat- and water-soluble vitamins simultaneously. The method has been validated in terms of detection limit, cut-off limit, sensitivity, number of false positives, number of false negatives and uncertainty range. The detection limit is about µg L-1. The screening method was applied to different samples such as pharmaceuticals, juices and isotonic drinks.
- Subjects
VITAMINS; CHLORIDES; SURFACE active agents; FLUORESCENCE; DRUGS
- Publication
Analytical & Bioanalytical Chemistry, 2005, Vol 381, Issue 8, p1568
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-005-3137-0