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- Title
A coumarin derivative covalently immobilized on sensing membrane as a fluorescent carrier for nitrofurazone.
- Authors
Jiao, Chen-Xu; Niu, Cheng-Gang; Chen, Li-Xin; Shen, Guo-Li; Yu, Ru-Qin
- Abstract
A coumarin derivative 4-methyl-8-methylacrylamide-2H, 5H-pyrano [3, 2-C] benzpyran 2, 5-dione (MMPBD) has been synthesized as a fluorescent carrier for preparing an optical chemical sensor. The carrier is immobilized on a quartz glass plate surface treated with a silanizing agent to prevent the leakage of the dye. This MMPBD sensor can be utilized for a nitrofurazone (NF) assay based on fluorescence quenching. The sensor shows good repeatability, a long lifetime and a fast response of less then 50 s. NF can be determined in the range between 1.0×10-6–1.0×10-3 mol L-1 with a detection limit of 8.0×10-7 mol L-1 at pH 7.0.
- Subjects
COUMARINS; BIOSENSORS; METAL quenching; NITROFURANS; FLUORESCENCE; COOLING
- Publication
Analytical & Bioanalytical Chemistry, 2003, Vol 376, Issue 3, p392
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-003-1909-y