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- Title
Allelic composition of MdMYB1 drives red skin color intensity in apple ( Malus × domestica Borkh.) and its application to breeding.
- Authors
Moriya, Shigeki; Kunihisa, Miyuki; Okada, Kazuma; Shimizu, Taku; Honda, Chikako; Yamamoto, Toshiya; Muranty, Hélène; Denancé, Caroline; Katayose, Yuichi; Iwata, Hiroyoshi; Abe, Kazuyuki
- Abstract
Since apple fruit skin reddens poorly under warmer climates, new apple cultivars are desired that are adapted to global warming in terms of bearing well-reddened fruit. We developed a simple sequence repeat marker, Mdo.chr9.4, which is suitable for red skin color selection. It amplified four alleles (Mdo.chr9.4-R, Mdo.chr9.4-Y, Mdo.chr9.4-Y, and Mdo.chr9.4-Y) distinguished by length. Mdo.chr9.4-R associated with MdMYB1- 1 which confers red fruit skin. The presence of Mdo.chr9.4-R was consistent with empirical skin color in all 160 tested accessions. Mdo.chr9.4 was identified as the only significant marker that contributed to red skin color intensity by a genome wide association study (GWAS), and it accounted for 52.0% of phenotypic variation, confirming that MdMYB1 was the major and principal determinant of fruit skin color in apples. Individuals with a homozygous state of Mdo.chr9.4-R (dose 2) were significantly redder than those showing a heterozygote state (dose 1) in both the accession set and full-sib families, indicating a partially dominant effect of MdMYB1- 1. Therefore, the selection of dose 2 individuals would target individuals with intensive red skin. We applied Mdo.chr9.4 to several application populations using a time and cost-efficient genotyping system developed in the present study. This system, along with Mdo.chr9.4, provide advanced marker-assisted breeding for intensive red skin color apples adapted to a global warming climate.
- Subjects
APPLES; PLANT breeding; COLOR of fruit; EFFECT of global warming on plants; NUCLEOTIDE sequencing; ALLELES in plants; PLANT genomes
- Publication
Euphytica, 2017, Vol 213, Issue 4, p1
- ISSN
0014-2336
- Publication type
Article
- DOI
10.1007/s10681-017-1864-x