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- Title
Three AFLP markers tightly linked to the genic male sterility ms<sub> 3</sub> gene in chili pepper ( Capsicum annuum L.) and conversion to a CAPS marker.
- Authors
Jundae Lee; Jae Bok Yoon; Jung-Heon Han; Won Phil Lee; Sang Hoon Kim; Hyo Guen Park
- Abstract
Genic male sterility (GMS) has long been used as a tool for hybrid seed production in chili pepper ( Capsicum annuum L.). We developed DNA markers linked to the GMS ms 3 gene in a segregating population using bulked segregant analysis (BSA) and amplified fragment length polymorphism (AFLP) techniques. The segregating population was subjected to BSA-AFLP with 512 primer combinations. Three AFLP markers (Eagg/Mccc276, Eagc/Mctt178, and Ecag/Mtgc204) were identified as tightly linked to the ms 3 locus. Among them, we converted the AFLP marker Ecag/Mtgc204 to the cleavage amplified polymorphic sequence (CAPS) marker, named GMS3-CAPS, based on sequencing analysis of internal and flanking regions for the markers between male-fertile and sterile plants. This marker will be useful for pepper breeding using the GMS system.
- Subjects
CAPSICUM annuum; GENETIC markers; PEPPERS; MALE sterility in plants; GENETIC polymorphisms
- Publication
Euphytica, 2010, Vol 173, Issue 1, p55
- ISSN
0014-2336
- Publication type
Article
- DOI
10.1007/s10681-009-0107-1