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- Title
Purification and characterization of novel extracellular endopolygalacturonases from a deep-sea yeast, Cryptococcus sp. N6, isolated from the Japan Trench.
- Authors
Miura, Takeshi; Abe, Fumiyoshi; Inoue, Akira; Usami, Ron; Horikoshi, Koki
- Abstract
Two novel endo-type polygalacturonases (PGase), with molecular weights of 36 kDa and 40 kDa (named p36 and p40, respectively), were purified from the supernatant of the culture medium of a deep-sea yeast, strain N6, isolated from the Japan Trench. The N-terminal 20 amino acids of p36 and p40 were identical, and the sequence homology was 47.4% in comparison with the PGase of Fusarium moniliforme. A treatment of p40 with glycopeptidase F reduced the molecular weight to 36 kDa, suggesting that p40 possessed N-acetylglucosamines on its asparagine residues and p40 might be matured by glycosylation of p36.
- Subjects
POLYGALACTURONASE; CULTURE media (Biology); MOLECULAR weights; AMINO acids; GLYCOSYLATION; GLYCOASPARAGINASE
- Publication
Biotechnology Letters, 2001, Vol 23, Issue 21, p1735
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1012488115482