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- Title
Improved Taxol production in suspension cultures of Taxus chinensis var. mairei by in situ extraction combined with precursor feeding and additional carbon source introduction in an airlift loop reactor.
- Authors
Ying-Jin Yuan; Zuo-Jun Wei; Zhao-Liang Wu; Jin-Chuan Wu
- Abstract
Taxus chinensis var. mairei was grown in two-liquid-phase culture in a 2.5 l airlift external loop reactor for production of Taxol. A mixture of oleic acid and dibutyl phthalate (1:1, v/v) in a volume ratio of 8:92 to the culture medium gave higher production of Taxol (up to 12 mg l-1) with an aeration rate of 1 vvm. By combination of the in situ extraction, precursor feeding and additional sugar introduction, Taxol production (16.7 mg l-1) was 5 times higher than that in the case without any additives (3.4 mg l-1) and 1.4 times of that in the case with only the in situ extraction (12 mg l-1). Taxol was extracted from the organic solvents with water and methanol (1:1:1.4, by vol.) and lyophilized giving a total yield of ca. 90%.
- Subjects
OLEIC acid; UNSATURATED fatty acids; PACLITAXEL; ALKALOIDS; DIBUTYL phthalate; PHTHALATE esters
- Publication
Biotechnology Letters, 2001, Vol 23, Issue 20, p1659
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1012483329863