We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Monotropein Protects Mesenchymal Stem Cells from Lipopolysaccharide-Induced Impairments and Promotes Fracture Healing in an Ovariectomized Mouse Model.
- Authors
Zhao, Shitian; Guo, Liqiang; Cui, Wei; Zhao, Yongjian; Wang, Jing; Sun, Kanghui; Zhang, Hong; Sun, Yueli; Zhao, Dongfeng; Hu, Xiaohui; Huang, Ziyu; Lu, Sheng; Wang, Yongjun; Liu, Xinhua; Zhang, Weian; Shu, Bing
- Abstract
Monotropein is one of the active ingredients in Morinda Officinalis, which has been used for the treatment in multiple bone and joint diseases. This study aimed to observe the in vitro effects of Monotropein on osteogenic differentiation of lipopolysaccharide treated bone marrow mesenchymal stem cells (bMSCs), and the in vivo effects of local application of Monotropein on bone fracture healing in ovariectomized mice. Lipopolysaccharide was used to set up the inflammatory model in bMSCs, which were treated by Monotropein. Molecular docking analysis was performed to evaluate the potential interaction between Monotropein and p65. Transverse fractures of middle tibias were established in ovariectomized mice, and Monotropein was locally applied to the fracture site using injectable hydrogel. Monotropein enhanced the ability of primary bMSCs in chondro-osteogenic differentiation. Furthermore, Monotropein rescued lipopolysaccharide-induced osteogenic differentiation impairment and inhibited lipopolysaccharide-induced p65 phosphorylation in primary bMSCs. Docking analysis showed that the binding activity of Monotropein and p65/14-3-3 complex is stronger than the selective inhibitor of NF-κB (p65), DP-005. Local application of Monotropein partially rescued the decreased bone mass and biomechanical properties of callus or healed tibias in ovariectomized mice. The expressions of Runx2, Osterix and Collagen I in the 2-week callus were partially restored in Monotropein-treated ovariectomized mice. Taking together, local application of Monotropein promoted fracture healing in ovariectomized mice. Inhibition of p65 phosphorylation and enhancement in osteogenesis of mesenchymal stem cells could be partial of the effective mechanisms.
- Subjects
MESENCHYMAL stem cells; FRACTURE healing; LABORATORY mice; ANIMAL disease models; BONE regeneration; JOINTS (Anatomy); SKIN regeneration
- Publication
Calcified Tissue International, 2023, Vol 113, Issue 5, p558
- ISSN
0171-967X
- Publication type
Article
- DOI
10.1007/s00223-023-01130-y