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- Title
Bulk preparation of (-)-epigallocatechin gallate-rich extract from green tea.
- Authors
Jian-Hui Ye; Na-Na Li; Jian-Liang Lu; Xin-Qiang Zheng; Yue-Rong Liang
- Abstract
(-)-Epigallocatechin gallate (EGCg)-rich extract (EGCg >700 mg g-1) was prepared from green tea leaves through a three-stage process consisting of liquid-liquid extraction and silica column purification. Crude tea extract was dissolved in ethyl acetate. After filtration, the solution was extracted by 10 gL-1 citric acid solution twice, and then passed through silica column. The catechins compounds in the ethyl acetate eluate were back extracted to the aqueous phase, then extracted with a mixed solution of n-hexane/ethyl acetate (2/5, v/v) 3 times, concentrated, and freeze dried. 12.8 g EGCg-rich extract containing 709 mg g-1 EGCg and 965 mg g-1 total catechins was obtained from 300 g green tea leaves, with an EGCg recovery of 26.1% and a yield of 4.3%. This method was suitable for bulk preparation of EGCg-rich catechins from green tea leaves.
- Subjects
EPIGALLOCATECHIN gallate; GREEN tea; PLANT extracts; ETHYL acetate; CATECHIN
- Publication
Food & Bioproducts Processing: Transactions of the Institution of Chemical Engineers Part C, 2014, Vol 92, Issue 3, p275
- ISSN
0960-3085
- Publication type
Article
- DOI
10.1016/j.fbp.2013.08.007