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- Title
HPLC 法测定灵芝孢子粉中三萜含量.
- Authors
杨志空; 韩伟; 冯娜; 张劲松; 王晨光; 周靖; 王金艳; 唐庆九
- Abstract
High-performance liquid chromatography method was established to determine the content of triterpenoids in Ganoderma lingzhi spore powder. 40% ethanol was proved to be the best solvent for extracting the medium-grade triterpenic acid in spore powder, and the extract was concentrated 50 times. The determination method of 13 standard products including ganoderic acid I, ganoderenic acid C, and ganoderic acid C2 were established by optimizing the column and elution conditions. The methodological investigation showed that RSD values of method precision, repeatability and stability were less than 5%, indicating the method was appropriate for the quantitative detection of medium-polar triterpenoids in Ganoderma lingzhi spore powder. The analysis of multiple samples showed that ganoderic acid C6, ganoderic acid G, ganoderic acid A, ganoderic acid D and ganoderic acid F were the main triterpenoids in Ganoderma lingzhi spore powder, and the content of ganoderic acid A was the highest, whose average proportion in the total quantity of triterpenoids was 19.71%. There was no correlation between triterpene content and wall breaking treatment. The content of triterpenoids in spore powder was 14.24–99.70μg/g, only accounting for one percent of triterpenoid content of the fruiting body. The content of triterpenoids in the Ganoderma lingzhi spore oil was less than 50μg/g. The results suggested that the content of triterpenoids in Ganoderma lingzhi spore powder and oil was too low to be regarded as the quantitative detection index of Ganoderma lingzhi spore powder products.
- Subjects
HIGH performance liquid chromatography; GANODERMA; SPORES; POWDERS; INVESTIGATIONS; TRITERPENOIDS
- Publication
Mycosystema, 2020, Vol 39, Issue 1, p184
- ISSN
1672-6472
- Publication type
Article
- DOI
10.13346/j.mycosystema.190196