We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Characterization of Hlcyst-3 as a member of cystatins from the tick Haemaphysalis longicornis.
- Authors
Zhou, Jinlin; Liao, Min; Gong, Haiyan; Xuan, Xuenan; Fujisaki, Kozo
- Abstract
The gene encoding cystatin from the tick Haemaphysalis longicornis has been reported previously. In the study reported here, we characterized a member of cystatins and designated it as Hlcyst-3 ( H. longicornis cystatin-3) . Its full-length cDNA is 602 bp, and it encodes a putative 129 amino acid protein with an obvious signal peptide. Sequence analysis revealed that it has significant homology with the known secreted cystatin. The recombinant protein was expressed in a GST-fused soluble form in Escherichia coli and was purified by affinity chromatography. The inhibitory activity of the recombinant protein against papain and cathepsin L was identified by fluorogenic substrate analysis. Real-time PCR revealed that Hlcyst-3 was mostly expressed in the tick midgut.
- Subjects
HAEMAPHYSALIS longicornis; CYSTATINS; RECOMBINANT proteins; ESCHERICHIA coli; AFFINITY chromatography
- Publication
Experimental & Applied Acarology, 2010, Vol 51, Issue 4, p327
- ISSN
0168-8162
- Publication type
Article
- DOI
10.1007/s10493-010-9336-1