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- Title
沙棘浆果中酚类物质含量与自由基 清除能力相关分析.
- Authors
陈道国; 张国昀; 张 彤; 张建国; 何彩云
- Abstract
[Objective] The relationship between hydroxyl radical scavenging capacity and seven phenolic substances in Seabuckthorn fruits was analyzed to provide reference for exploring the hydroxyl radical scavenging capacity of phenolic substances. [Method] The sea buckthorn fruits of Fengning cultivar and Xiangyang cultivar in three development stages(green fruit stage, discoloration stage and mature stage) were used as experimental materials. The contents of seven phenolic substances such as rutin, quercetin, isorhamnetin, kaempferol, myricetin, naringenin and vitamin E were determined by gas chromatography-mass spectrometry(GC-MS) technique. The hydroxyl radical scavenging ability of sea buckthorn fruit was determined by spectrophotometry. Then the correlation between the seven phenolic substances and hydroxyl radical scavenging capacity was analyzed. [Result] The results showed that the hydroxyl radical scavenging ability of Fengning cultivar was significantly higher than that of Xiangyang cultivar, the hydroxyl radical scavenging ability of Fengning cultivar decreased successively in the three development stages, while that of Fengning cultivar was the strongest in green fruit stage and the weakest in discoloration stage. The contents of rutin, quercetin, isorhamnetin, kaempferol, myricetin and naringenin in the sarcocarp of Fengning cultivar were higher than those in Xiangyang cultivar. The contents of rutin and myricetin in the sarcocarp of Fengning cultivar were significantly higher than those in Xiangyang cultivar during the three stages(P<0.05). In addition, the contents of quercetin, isorhamnetin, naringenin and myricetin increased at first and then decreased in the three developmental stages, while the changes of rutin, vitamin E and kaempferol were different between the two cultivars. The correlation analysis showed that the correlation coefficients between rutin, quercetin, isorhamnetin, kaempferol, myricetin and naringenin and hydroxyl radical scavenging ability were all greater than 0.6, while the correlation coefficients between vitamin E and hydroxyl radical scavenging ability were only 0.17. [Conclusion] The hydroxyl radical scavenging ability are strongly correlated with the contents of rutin, quercetin, isorhamnetin, kaempferol, myricetin and naringenin, which were helpful to promote the scavenging of hydroxyl radicals in seabuckthorn fruit
- Subjects
HYDROXYL group; VITAMIN E; SEA buckthorn; FLAVONOLS; MYRICETIN; QUERCETIN
- Publication
Forest Research, 2019, Vol 32, Issue 5, p27
- ISSN
1001-1498
- Publication type
Article
- DOI
10.13275/j.cnki.lykxyj.2019.05.004