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- Title
Self-Incompatibility in Petunia inflata: The Relationship between a Self-Incompatibility Locus F-Box Protein and Its Non-Self S-RNases.
- Authors
Sun, Penglin; Kao, Teh-hui
- Abstract
The highly polymorphic S (for self-incompatibility) locus regulates self-incompatibility in Petunia inflata ; the S-RNase regulates pistil specificity, and multiple S-locus F-box (SLF) genes regulate pollen specificity. The collaborative non-self recognition model predicts that, for any S -haplotype, an unknown number of SLFs collectively recognize all non-self S-RNases to mediate their ubiquitination and degradation. Using a gain-of-function assay, we examined the relationships between S2-SLF1 (for S2 -allelic product of Type-1 SLF) and four S-RNases. The results suggest that S2-SLF1 interacts with S7- and S13-RNases, and the previously identified S1- and S3-RNases, but not with S5- or S11-RNase. An artificial microRNA expressed by the S2-SLF1 promoter, but not by the vegetative cell-specific promoter, Late Anther Tomato 52 , suppressed expression of S2-SLF1 in S2 pollen, suggesting that SLF1 is specific to the generative cell. The S2 pollen with S2 - SLF1 suppressed was compatible with S3 -, S5 -, S7 -, S11 -, and S13 -carrying pistils, confirming that other SLF proteins are responsible for detoxifying S5- and S11-RNases and suggesting that S2-SLF1 is not the only SLF in S2 pollen that interacts with S3-, S7-, and S13-RNases. Petunia may have evolved at least two types of SLF proteins to detoxify any non-self S-RNase to minimize the deleterious effects of mutation in any SLF.
- Subjects
PETUNIAS; LOCUS (Genetics); PISTIL; PROTEINS; POLLEN
- Publication
Plant Cell, 2013, Vol 25, Issue 2, p470
- ISSN
1040-4651
- Publication type
Article
- DOI
10.1105/tpc.112.106294