We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Subgenome Discrimination in Brassica and Raphanus Allopolyploids Using Microsatellites.
- Authors
Campomayor, Nicole Bon; Waminal, Nomar Espinosa; Kang, Byung Yong; Nguyen, Thi Hong; Lee, Soo-Seong; Huh, Jin Hoe; Kim, Hyun Hee
- Abstract
Intergeneric crosses between Brassica species and Raphanus sativus have produced crops with prominent shoot and root systems of Brassica and R. sativus, respectively. It is necessary to discriminate donor genomes when studying cytogenetic stability in distant crosses to identify homologous chromosome pairing, and microsatellite repeats have been used to discriminate subgenomes in allopolyploids. To identify genome-specific microsatellites, we explored the microsatellite content in three Brassica species (B. rapa, AA, B. oleracea, CC, and B. nigra, BB) and R. sativus (RR) genomes, and validated their genome specificity by fluorescence in situ hybridization. We identified three microsatellites showing A, C, and B/R genome specificity. ACBR_msat14 and ACBR_msat20 were detected in the A and C chromosomes, respectively, and ACBR_msat01 was detected in B and R genomes. However, we did not find a microsatellite that discriminated the B and R genomes. The localization of ACBR_msat20 in the 45S rDNA array in ×Brassicoraphanus 977 corroborated the association of the 45S rDNA array with genome rearrangement. Along with the rDNA and telomeric repeat probes, these microsatellites enabled the easy identification of homologous chromosomes. These data demonstrate the utility of microsatellites as probes in identifying subgenomes within closely related Brassica and Raphanus species for the analysis of genetic stability of new synthetic polyploids of these genomes.
- Subjects
RUTABAGA; MICROSATELLITE repeats; RADISHES; COLE crops; HOMOLOGOUS chromosomes; FLUORESCENCE in situ hybridization; BRASSICA
- Publication
Cells (2073-4409), 2021, Vol 10, Issue 9, p2358
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells10092358