We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Selection of Putative Polyester Hydrolases from the Metagenome of Los Humeros Geothermal Field by Means of In Silico Probes.
- Authors
Solis-Palacios, Rocio; Espinosa-Luna, Graciela; Peña-Montes, Carolina; Quintana-Castro, Rodolfo; Sánchez-Otero, María Guadalupe; Oliart-Ros, Rosa María
- Abstract
Hydrolases are the most popular enzymes, and among the most valuable in biotechnological applications. Some hydrolases, such as lipases, esterases, proteases, cellulases and amylases, are used in the food industry and the production of biopharmaceuticals, biofuels, biopolymers and detergents. Of special interest are those obtained from thermophilic microorganisms. Although there is great microbial diversity in extreme environments, the investigations aimed at detecting and isolating enzymes with potential for polyester degradation such as polyethylene terephthalate (PET) are limited. In this work, we explored the metagenomic library of an oil-enriched soil sample from the "Los Humeros" geothermal field by means of in silico probes in search for enzymes potentially able to degrade polyesters. Using conserved motifs and activity-relevant sites of reported polyester hydrolases, we designed probes that allowed us to identify 6 potential polyester hydrolases in the metagenome. Three-dimensional structure prediction revealed a canonical α/β fold and a cap covering the active site of the enzymes. The catalytic triads were composed of Ser, His and Asp. Structural comparison, substrate binding site analysis and molecular docking suggested their potential as polyester hydrolases, particularly cutinases and PETases. An enzyme, REC98271, was cloned, expressed and characterized, showing thermophilic properties and preference for short-chain substrates. These findings contribute to our understanding of enzyme diversity in "Los Humeros" metagenome and their potential applications in biodegradation and recycling processes.
- Subjects
HYDROLASES; BINDING site assay; BINDING sites; POLYESTERS; POLYETHYLENE terephthalate; LIPASES
- Publication
Catalysts (2073-4344), 2024, Vol 14, Issue 6, p379
- ISSN
2073-4344
- Publication type
Article
- DOI
10.3390/catal14060379