We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
APPLICATION OF REAL-TIME PCR, NESTED-PCR AND MYCOLOGY ASSAY USING BAL SAMPLES FOR DETECTION OF ASPERGILLUS FLAVUS AND A. FUMIGATUS IN HEMATOLOGICAL MALIGNANCIES.
- Authors
Zarrinfar, Hossein; Mirhendi, Hossein; Fata, Abdolmajid; Makimura, Koichi; Paknejhad, Omolbanin; Saber, Sasan; Khodadadi, Hossein; Satoh, Kazuo; Kordbacheh, Parivash
- Abstract
Introduction and Objectives: Invasive aspergillosis (IA) represents a major cause of morbidity and mortality in patients with hematological malignancies. IA is caused by several pathogenic Aspergillus species, mainly A. fumigatus and A. flavus. To evaluate four diagnostic methods: direct microscopy, culture, nested-PCR and real-time PCR for detection of A. fumigatus and A. flavus and to investigate the incidence of pulmonary aspergillosis (PA) on bronchoalveolar lavage (BAL) samples in patients with hematological malignancies in Tehran, Iran. Materials and Methods: During 16 months, 28 BAL samples from patients with hematological malignancies were obtained. Direct wet mounts on %20 KOH and culture on mycological media were performed. The nested-PCR detection of A. fumigatus and A. flavus was based on conserved sequences of internal transcribed spacer 1 (ITS1) ribosomal DNA and the real-time PCR assay targeted β-tubulin gene. Results: Of the 28 samples, 2 (7. 1%) were positive in direct examination having branching septate hyphae, 6 (21. 4%) had positive culture with 5 (83%) A. flavus and 1 (17%) A. fumigatus, 12 (42. 9%) were positive in nested-PCR and 5 (17. 9%) had positive real-time PCR. The incidence of aspergillosis in these patients included probable IPA in 6 (21. 5%), possible IPA in 13 (46. 5%) and not IPA in 9 (32%). A. flavus was the most common cause of PA. Conclusion: Because of high sensitivity of nested- PCR, it may increase the false positive result due to fungal contamination and colonization; therefore, it is not recommend for BAL samples in diagnosis of PA. However, in high risk patients, a positive result of real-time PCR or nested-PCR corresponding with clinical signs or symptoms could be valuable.
- Subjects
POLYMERASE chain reaction; ASPERGILLUS flavus; PULMONARY aspergillosis; MYCOLOGY; ASPERGILLUS fumigatus; RIBOSOMAL DNA; PATIENTS
- Publication
Jundishapur Journal of Microbiology, 2013, p13
- ISSN
2008-3645
- Publication type
Article