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- Title
Association between NET-1 Level in Placenta from Preeclampsia Pregnancies and Trophoblast Proliferation and Apoptosis: A Correlation Analysis.
- Authors
Shu Luo; Ying Hu; Shuming Li; Linang Wang; Yong Huang
- Abstract
Objective • This study investigates the expression pattern of neuroepithelial cell transforming 1 (NET-1) in placental tissues from pregnancies with preeclampsia (PE) and explores its role in mediating proliferative and apoptotic capacities of trophoblasts. Methods • The relative mRNA levels of NET-1 in placental tissues obtained from preeclampsia (PE) pregnancies (n=60) and healthy pregnancies (n=60) were determined using quantitative real-time polymerase chain reaction (qRT-PCR). Pearson correlation test was conducted to assess the correlation between NET-1 level and systolic (Sp) and diastolic pressure (Dp) in PE pregnancies. After the knockdown of NET-1 in HTR-8/SVneo cells, changes in proliferation and apoptosis were examined using cell counting kit-8 (CCK-8) and flow cytometry, respectively. Results • NET-1 was highly expressed in placental tissues from PE pregnancies. PE patients with a high level of NET-1 had higher Sp and Dp, and NET-1 level was positively correlated with both Sp and Dp. Knockdown of NET-1 in HTR-8/SVneo cells decreased the proliferative rate but increased the apoptotic rate. Conclusions • NET-1 stimulates the development of PE by triggering trophoblast proliferation and inhibiting apoptosis. Therefore, NET-1 could be a potential therapeutic target for treating PE and other related hypertensive disorders during pregnancy.
- Subjects
PLACENTA; PREECLAMPSIA; POLYMERASE chain reaction; APOPTOSIS; TROPHOBLAST
- Publication
Alternative Therapies in Health & Medicine, 2023, Vol 29, Issue 5, p353
- ISSN
1078-6791
- Publication type
Article