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- Title
Molecular characterization of nitrate reductase gene and its expression in the marine red alga Gracilaria tenuistipitata (Rhodophyta).
- Authors
Falcão, Vanessa R.; Oliveira, Mariana C.; Colepicolo, Pio
- Abstract
The enzyme nitrate reductase (NR) responsible for the conversion of nitrate to nitrite is considered to be the rate-limiting step in nitrogen assimilation. The economically important marine macroalga Gracilaria tenuistipitata presents a circadian oscillation in NR protein content and activity. In order to identify if the regulation of NR in G. tenuistipitata happens at transcriptional levels, the NR cDNA and gene were sequenced and the NR mRNA expression was studied. Analysis of the sequenced gene revealed absence of introns which is unusual for NR genes. The transcriptional profiling revealed a circadian rhythm for NR; furthermore, a rhythm was observed in constant light condition, suggesting a possible regulation by the biological clock at the mRNA levels for NR in G. tenuistipitata.
- Subjects
ENZYMES; NITRATES; ALGAE; DNA; GENETIC transcription; CIRCADIAN rhythms
- Publication
Journal of Applied Phycology, 2010, Vol 22, Issue 5, p613
- ISSN
0921-8971
- Publication type
Article
- DOI
10.1007/s10811-010-9501-2