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- Title
Expression profiles of genes associated with viral entry in HCV-infected human liver.
- Authors
Nakamuta, M.; Fujino, T.; Yada, R.; Aoyagi, Y.; Yasutake, K.; Kohjima, M.; Fukuizumi, K.; Yoshimoto, T.; Harada, N.; Yada, M.; Kato, M.; Kotoh, K.; Taketomi, A.; Maehara, Y.; Nakashima, M.; Enjoji, M.
- Abstract
Recent studies have demonstrated that several cellular factors are involved in entry of hepatitis C virus (HCV) into host cells. Detailed gene expression profiles of these factors in HCV-infected livers have not been reported for humans. Transcriptional levels of LDL receptor (LDLR), CD81, scavenger receptor class B type I (SR-BI), claudin-1, and occludin genes in liver samples from patients with chronic hepatitis C were investigated. Serum levels of LDL-cholesterol (LDL-C) and HCV core antigen were also evaluated, and expression of claudin-1 and occludin were immunohistochemically analyzed. Compared with normal liver, transcription of LDLR and claudin-1 genes was significantly suppressed ( P < 0.0001) and occludin transcription was significantly up-regulated in HCV-infected livers ( P < 0.0001). Significant positive correlations were found for LDLR versus occludin, LDLR versus claudin-1, occludin versus claudin-1, and CD81 versus SR-BI in HCV-infected ( P = 0.0012, P < 0.0001, P = 0.0004, and P < 0.0001, respectively) and normal livers ( P < 0.0001, P = 0.0051, P < 0.0001, and P < 0.0001, respectively). Positive correlation was observed between serum levels of HCV core antigen and LDL-C ( P = 0.0147), with their levels negatively correlated to LDLR ( P = 0.0270 and P = 0.0021, respectively). Immunohistochemically, hepatocellular expression of claudin-1 and occludin was increased in HCV-infected livers. Different levels of expression were demonstrated at the mRNA and protein levels for occludin and claudin-1 in HCV-infected and normal livers. Correlation of elements associated with viral entry was comparable in HCV-infected and normal livers. J. Med. Virol. 83:921-927, 2011. © 2011 Wiley-Liss, Inc.
- Publication
Journal of Medical Virology, 2011, Vol 83, Issue 5, p921
- ISSN
0146-6615
- Publication type
Article
- DOI
10.1002/jmv.22042