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- Title
α-Lipoic acid ameliorates impaired glucose uptake in LYRM1 overexpressing 3T3-L1 adipocytes through the IRS-1/Akt signaling pathway.
- Authors
Qin, Zhen-Ying; Zhang, Min; Guo, Xi-Rong; Wang, Yu-Mei; Zhu, Guan-Zhong; Ni, Yu-Hui; Zhao, Ya-Ping; Qiu, Jie; Kou, Chun-Zhao; Qin, Rui; Cao, Xin-Guo
- Abstract
Overexpression of the Homo sapiens LYR motif containing 1 (LYRM1) causes mitochondrial dysfunction and induces insulin resistance in 3T3-L1 adipocytes. α-Lipoic acid (α-LA), a dithiol compound with antioxidant properties, improves glucose transport and utilization in 3T3-L1 adipocytes. The aim of this study was to investigate the direct effects of α-LA on reactive oxygen species (ROS) production and insulin sensitivity in LYRM1 overexpressing 3T3-L1 adipocytes and to explore the underlying mechanism. Pretreatment with α-LA significantly increased both basal and insulin-stimulated glucose uptake and insulin-stimulated GLUT4 translocation, while intracellular ROS levels in LYRM1 overexpressing 3T3-L1 adipocytes were decreased. These changes were accompanied by a marked upregulation in expression of insulin-stimulated tyrosine phosphorylation of IRS-1 and serine phosphorylation of Akt following treatment with α-LA. These results indicated that α-LA protects 3T3-L1 adipocytes from LYRM1-induced insulin resistance partially via its capacity to restore mitochondrial function and/or increase phosphorylation of IRS-1 and Akt.
- Subjects
OBESITY treatment; LIPOIC acid; FAT cells; GENETIC regulation; INSULIN resistance; CELLULAR signal transduction; GLUCOSE transporters
- Publication
Journal of Bioenergetics & Biomembranes, 2012, Vol 44, Issue 5, p579
- ISSN
0145-479X
- Publication type
Article
- DOI
10.1007/s10863-012-9460-1