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- Title
The abp gene in Geobacillus stearothermophilus T-6 encodes a GH27 β-l-arabinopyranosidase
- Authors
Salama, Rachel; Alalouf, Onit; Tabachnikov, Orly; Zolotnitsky, Gennady; Shoham, Gil; Shoham, Yuval
- Abstract
Abstract: In this study we demonstrate that the abp gene in Geobacillus stearothermophilus T-6 encodes a family 27 glycoside hydrolase β-l-arabinopyranosidase. The catalytic constants towards the chromogenic substrate pNP-β-l-arabinopyranoside were 0.8±0.1mM, 6.6±0.3s−1, and 8.2±0.3s−1 mM−1 for K m, k cat and k cat /K m, respectively. 13C NMR spectroscopy unequivocally showed that Abp is capable of removing β-l-arabinopyranose residues from the natural arabino-polysaccharide, larch arabinogalactan. Most family 27 enzymes are active on galactose and contain a conserved Asp residue, whereas in Abp this residue is Ile67, which shifts the specificity of the enzyme towards arabinopyranoside.
- Subjects
GEOBACILLUS stearothermophilus; PYRANOSIDE; BACTERIAL enzymes; BACTERIAL genes; GENETIC code; NUCLEAR magnetic resonance spectroscopy; CHROMOGENIC compounds
- Publication
FEBS Letters, 2012, Vol 586, Issue 16, p2436
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2012.05.062