We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
The SR-rich motif in SARS-CoV nucleocapsid protein is important for virus replication.
- Authors
Tylor, Shaun; Andonov, Anton; Cutts, Todd; Cao, Jingxin; Grudesky, Elsey; Van Domselaar, Gary; Li, Xuguang; He, Runtao
- Abstract
The multimerization/self-interaction of viral proteins is an important step in the process of viral assembly and maturation. Our previous study indicated that the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid protein forms self-multimers through a serine-arginine (SR)-rich motif (SSRSSSRSRGNSR) by using a mammalian two-hybrid system. To determine the biological relevance of this motif, we constructed a SARS-CoV reverse genetic construct by using a bacterial artificial chromosome (BAC)-based vector controlled by a T7 promoter; and subsequently deleted the SR-rich motif from the N gene. The mutated infectious clone showed reduced level of genome transcription and significantly reduced levels of the infectious virions. These results strongly suggest that the SR-rich motif is critical for effective virus replication.
- Subjects
VIRAL load; VIRAL proteins; SARS disease; CORONAVIRUSES; GENOMES; GENES; SERINE; ARGININE; PATHOGENIC microorganisms
- Publication
Canadian Journal of Microbiology, 2009, Vol 55, Issue 3, p254
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/W08-139